Reverse Transcriptase, Recombinant, HIV from Worthington Biochemical Corporation

Product Specs
ItemReverse Transcriptase, Recombinant, HIV
CompanyWorthington Biochemical Corporation
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberLS05006
Source/Expression SystemE. coli plasmid pRC-RT
StorageStore at -20°C
Activity≥10,000 units per mg protein
Quantity5x200 un

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Worthington Biochemical Corporation

Lakewood, New Jersey 8701

United States

Phone: 1.800.445.9603
1.732.942.1660

Email: custservice@worthington-biochem.com

Company Profile

Website: worthington-biochem.com

(1) Host cell glycosylation selects for infection with CCR5- versus CXCR4-tropic HIV-1bioRxivSeptember 9, 2023Hannah L. Itell, Daryl Humes, Nell E. Baumgarten, Julie Overbaughdetermined multiple times using a standard curve of purified HIV RT enzyme (Worthington Biochemical Corp. #LS05003). Aliquots of this titered stock of Q23.BG505 were used as the quantitative standard curve in all assays.

(2) m6A-SAC-seq for quantitative whole transcriptome m6A profilingNature ProtocolsFebruary 1, 2023Ruiqi Ge, Chang Ye, Yong Peng, Qing Dai, Yutao Zhao, Shun Liu, Pingluan Wang, Lulu Hu, Chuan HeNew England BioLabs, cat. no. M0277S) Reverse Transcriptase, Recombinant HIV (Worthington Biochemical, cat. no. LS05006) Actinomycin D (C62H86N12O16, Sigma-Aldrich, cat. no. A1410) Deoxynucleotide (dNTP) Solution Mix

(3) REverSe TRanscrIptase chain termination ( RESTRICT ) for selective measurement of nucleotide analogs used in HIV care and preventionBioengineering & Translational MedicineJanuary 1, 2023Ayokunle O. Olanrewaju, Benjamin P. Sullivan, Alicia H. Gim, Cosette A. Craig, Derin Sevenler, Andrew T. Bender, Paul K. Drain, Jonathan D. PosnerOrganics, Fair Lawn, NJ). Recombinant RT enzyme was obtained from Worthington Biochemical Corporation (LS05006, Lakewood, NJ). The nucleotide analogs TFV‐DP (166403‐66‐3 sodium salt), azidothymidine‐5′‐triphosphate

(4) Analysis of queuosine and 2-thio tRNA modifications by high throughput sequencingNucleic Acids ResearchSeptember 23, 2022Christopher D Katanski, Christopher P Watkins, Wen Zhang, Matthew Reyer, Samuel Miller, Tao PanThe enzymes used were AffinityScript (Agilent 600105), HIV-RT (Worthington Biochemical Corporation, LS05003), MuMLV (NEB M0253L), RevertAid (ThermoFisher EP0441), SuperScript III (ThermoFisher 18080093), and

(5) m6A-label-seq: A metabolic labeling protocol to detect transcriptome-wide mRNA N6-methyladenosine (m6A) at base resolutionSTAR ProtocolsMarch 1, 2022Xiao Shu, Jie Cao, Jianzhao LiuTrizol Reagent,Invitrogen,Cat#15596018 Reverse transcriptase recombinant HIV,Worthington Biochemical Corporation,Cat#LS05003 Reverse transcriptase RevertAid,Thermo Scientific,Cat#EP0441 5x RT reaction buffer,Thermo Scientific,Cat#EP0441 Recombinant

(6) RNase H sequence preferences influence antisense oligonucleotide efficiencyNucleic Acids ResearchDecember 15, 2017Łukasz J. Kiełpiński, Peter H. Hagedorn, Morten Lindow, Jeppe Vinther0.5 mU/μl. Reactions with HIV-1 Reverse Transcriptase from Worthington Biochemical Corporations (cat. LS05003) were performed as human RNase H1, with the final concentration of the enzyme 0.1 U/μl. Control hydrolysis

(7) Evaluation of the Broad-Range PCR-Electrospray Ionization Mass Spectrometry (PCR/ESI-MS) System and Virus Microarrays for Virus DetectionVirusesApril 25, 2014Lanyn P. Taliaferro, Teresa A. Galvin, Hailun Ma, Syed Shaheduzzaman, Dhanya K. Williams, Dustin R. Glasner, Arifa S. Khanstandard curve using HIV-1 RT enzyme (Worthington Biochemical Corporation Lakewood, NJ, USA, cat. No. LS05006; lot No. X1H2839), which was determined to have 262 pU of RT activity per particle [40]. The STF-PERT

(8) Enzymatic synthesis of DNA strands containing α-L-LNA (α-L-configured locked nucleic acid) thymine nucleotidesArtificial DNA: PNA & XNAJune 9, 2012Torben Højland, Rakesh N. Veedu, Birte Vester, Jesper WengelmM) 0.60 ml Dpo4 (0.40 mg/ml) 12.3 ml Twice distilled water HIV RT (Worthington biochemical company, LS05003). 4.00 ml 5X HIV RT buffer (250 mM TRIS-HCl, 300 mM KCl, 12.5 mM MgCl2) 1.50 ml Triphosphate mixture

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Reverse Transcriptase, Recombinant, HIV from Worthington Biochemical Corporation

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Worthington Biochemical Corporation

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Reverse Transcriptase, Recombinant, HIV from Worthington Biochemical Corporation

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